
Additionally, the different expressions of eight LcDFRs and four LcANS genes were positively correlated with flavonoid biosynthesis, and the four LcBZ1 as well as one Lc3Mat1 were positively correlated with the content of seven anthocyanins revealed by coupling with metabolomics and transcriptomics analyses. Combined with NGS analyses, the DETs involved in flavonoids and anthocyanins contributed greatest to the flower coloring.

Among them, 52,851 transcripts were annotated in the seven databases of NR, KOG, GO, NT, Pfam, Swiss-Port, and KEGG. rubrum was built, which had 171,783 high-quality nonredundant transcripts with correcting with next-generation sequencing (NGS). Meanwhile, the first reference full-length transcriptome of L. Of these, 13 flavonoid compounds were considered significantly different expression compounds highly consistent with color information in the 4 samples. Moreover, a total of 207 flavonoid components were identified.

The total anthocyanins in addition to the RHSCC values and CIE 1976 L*a*b* values of petals were highly consistent with petal color. Here, we profiled the flavonoid metabolome and carried out full-length sequencing in addition to transcriptome analyses to investigate the flavonoid biosynthesis and global transcriptome changes among different petal coloring cultivars of L. rubrum are typical as well as traditional ornamental and Chinese herbal medicines in Asia however, more information is needed on the mechanisms underlying their flower coloring. The Loropetalum chinense and Loropetalum chinense var.
